Cytoplasmic 5'(3')-nucleotidase from human placenta.
نویسندگان
چکیده
منابع مشابه
Human placental cytoplasmic 5'-nucleotidase. Kinetic properties and inhibition.
The kinetic properties of highly purified human placental cytoplasmic 5'-nucleotidase were investigated. Initial velocity studies gave Michaelis constants for AMP, IMP, and CMP of 18, 30, and 2.2 microM, respectively. The enzyme shows the following relative Vmax values: CMP greater than UMP greater than dUMP greater than GMP greater than AMP greater than dCMP greater than IMP. The activity was ...
متن کاملHigh Km soluble 5'-nucleotidase from human placenta. Properties and allosteric regulation by IMP and ATP.
A human placental soluble "high Km" 5'-nucleotidase has been separated from "low Km" 5'-nucleotidase and nonspecific phosphatase by AMP-Sepharose affinity chromatography. The enzyme was purified 8000-fold to a specific activity of 25.6 mumol/min/mg. The subunit molecular mass is 53 kDa, and the native molecular mass is 210 kDa, suggesting a tetrameric structure. Soluble high Km 5'-nucleotidase ...
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The method of countercurrent fractionation has been applied to the separation of a wide variety of substances. Among animal lipids, Lovern (1952) has studied ox-brain phospholipids, Lovern & Olley (1952) fractionated haddock-muscle lipids, while the lipids of human brain have been investigated by Cole, Lathe & Ruthven (1953). The latter study has now been extended to the lipid components of hum...
متن کاملStructure-activity relationship of cytoplasmic 5'-nucleotidase substrate sites.
Various 5'-nucleotidases (EC 3.1.3.5) exist in vertebrate tissues. The sequence and cDNA cloning of the membrane-bound ecto-5'-nucleotidase (e-N) and one of the cytosolic isoenzymes, IMP-preferring (c-N-II), but not the cytosolic AMP-preferring form (c-N-I), have been reported. While c-N-II has a broad tissue distribution, c-N-I is found only in vertebrate heart. The published data on substrate...
متن کاملHuman Mesenchymal Stem Cells Derived from Adiopose Tissue and Placenta and the Adipocytic and Osteocytic Differentiation
Introduction: Mesenchymal stem cells can be isolated from adult tissues, such as the adipose tissue, or other sources. Among all these sources, adipose tissue because of easy access, and placenta due to its immunomodulatory properties, in addition to another useful properties, were attracted more attention to themselves. Isolation and comparing these two different sources can help us for acces...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1990
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)39188-4